What is the best way to choose an antibody for my CUT&Tag assay if I can not find a CUT&Tag-validated antibody for my target protein?

We always recommend starting with CST CUT&Tag-validated antibodies because CST scientists perform intensive in-house tests to exclude any antibody that tends to show non-specific tagmentation at open chromatin regions (a unique problem for the CUT&Tag assay). If a CUT&Tag-validated antibody is not available, we recommend starting with a CUT&RUN-validated antibody. Alternatively, you may want to try an antibody validated for ChIP, ChIP-seq, or an immunofluorescence assay. The antibody binding in CUT&Tag takes place in an intact nuclear environment, resembling conditions for antibody binding in immunofluorescence assays. If a non-CUT&Tag-validated antibody is used, it is imperative to confirm that there is no non-specific tagmentation, otherwise your results might not be reliable.

Last updated: September 12, 2024