What is the cross-reactivity of your Myosin Light Chain 2 antibodies with Myosin 12a/b?

We have not tested the reactivity of our myosin light chain 2 antibodies against myosin 12a/b (UniProt: P19105 and O14950). However, based on homology, there is a good chance for cross-reactivity. The length of MLC12a/b is 171 amino acids and 172 amino acids respectively and both are predicted to run at ~20 kDa.

The peptide immunogens used to produce our Myosin Light Chain 2 antibodies have the following sequence homology with Myosin 12a and 12b:

• Myosin Light Chain 2 Antibody #3672: 77% homology with Myosin 12a, 85% homology with Myosin 12b
• Myosin Light Chain 2 (D18E2) Rabbit mAb #8505: 92% homology with both Myosin 12a and 12b
• Phospho-Myosin Light Chain 2 (Ser19) Antibody #3671: 100% homology with both Myosin 12a and 12b
• Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody #3674: 100% homology with both Myosin 12a and 12b
• Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb #3675: 100% homology with both Myosin 12a and 12b
• Phospho-Myosin Light Chain 2 (Thr18/Ser19) (E2J8F) Rabbit mAb #95777: 100% homology with both Myosin 12a and 12b

Last updated: February 26, 2024