What is the difference between Normal Rabbit IgG #2729 and Rabbit (DA1E) mAb IgG XP® Isotype Control #3900?

Our Normal Rabbit IgG #2729 is an unconjugated rabbit polyclonal antibody that is routinely used as a non-specific rabbit IgG control for IP and ChIP, whereas our Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 is an unconjugated rabbit monoclonal antibody that is routinely used as a non-specific rabbit IgG control for IP, IHC-P, IF-IC, F, and ChIP.

Which of the two is recommended depends on the application:

IP:

#3900 is recommended as a negative control for IP experiments with rabbit monoclonal antibodies, whereas #2729 is recommended as a negative control for IP experiments with rabbit polyclonal antibodies.

IF and Flow: 

#3900 is recommended as a negative control for IF and flow cytometry experiments with both rabbit polyclonal and rabbit monoclonal antibodies.

IHC:

#3900 is recommended as a negative control for IHC experiments with rabbit monoclonal antibodies, whereas Jackson ChromPure IgG (#011-000-003; https://www.jacksonimmuno.com/catalog/products/011-000-003) is recommended as a negative control for IHC experiments with rabbit polyclonal antibodies.

ChIP:

Both #2729 and #3900 work well as negative rabbit IgG controls for ChIP assays. However, #2729 can sometimes pull down more non-specific background than #3900. Therefore, we prefer to use #2729 for our in-house tests as a stricter negative control.


 

Last updated: September 12, 2024