What is the difference between phospho-Smad2 (Ser465/467) and phospho-Smad2 (Ser245/250/255)?

Phosphorylation of serines 465 and 467 indicates Smad2 activation and nuclear localization; whereas, phosphorylation of serines 245, 250, and 255 is inhibitory and leads to retention of Smad2 in the cytoplasm.

Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108, Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828, and Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit mAb #18338 recognize phosphosites at the carboxy-terminus of Smad2 that are phosphorylated in response to TGF-beta treatment. Phospho-Smad2 (Ser245/250/255) Antibody #3104 instead recognizes a cluster of phosphosites in the Smad2 linker region that are phosphorylated by MAP kinase.  
 

Last updated: March 14, 2024