What is the minimal amount of tissue required for a CUT&RUN experiment?

While we recommend 1mg of tissue per assay in most cases, we have had some success with as little as 0.5 mg of tissue per assay. Easier tissue types, such as liver, or easier target types, such as histone modifications, require less tissue. Difficult tissue types like heart or difficult target types like some transcription factors or cofactors will likely require greater amounts of tissue, such as 2.5mg or 5mg.

 

Last updated: February 28, 2024