What procedure do you recommend for antigen retrieval with citrate?

We recommend using a microwave to bring slides to a boil in 10 mM sodium citrate buffer, pH 6.0 and then to maintain at sub-boiling temperature for 10 minutes. This typically requires heating slides at full power for 2.5 -3 minutes, then adjusting to 20-30% power.  After microwave heating, we cool the slides on the bench top for 30 minutes.

Last updated: September 12, 2024