What secondary antibody should I use to detect a chimeric antibody?

To detect a recombinant chimeric monoclonal antibody, use a species-specific secondary antibody. For example, you would use an anti-feline secondary antibody to detect a chimeric antibody generated with a feline Fc domain. 

When multiplexing, it is important to note that these chimeric antibodies retain the antigen-binding Fab region of their original parent host antibody. You can find additional product-specific details in the "Source/Purification" section on the product webpage or datasheet.

Fc-directed secondary antibodies are required to detect primary antibodies whose host species match the chimeric antibody’s parent host species. For instance, if you are working with a feline chimeric antibody with a rabbit host species and a rabbit primary antibody, use an anti-feline secondary antibody for the chimeric antibody and an Fc-directed anti-rabbit secondary antibody for the rabbit primary antibody. Using non-Fc-directed secondary antibodies may result in cross-reactivity with the chimeric antibody due to its retained Fab region. Secondary antibodies can be combined into a cocktail for more efficient application to samples.

 

Last updated: June 2, 2025