What will happen if you forget to add the FastScan™ ELISA Cell Extraction Enhancer Solution (50X) #25243 to your lysates?

Depending on the target, the FastScan™ ELISA Cell Extraction Enhancer Solution #25243 usually increases the sensitivity/signal and performance of the kit. Therefore, if it is omitted, your signal may be lower, however the extent of this effect will vary from kit to kit. We recommend using the enhancer to avoid this possibility. If you forgot to add the enhancer to the FastScan™ ELISA Cell Extraction Buffer #69905 that was used to make the sample lysate, you can spike in the 50x Enhancer solution afterwards (and always add the enhancer to the extraction buffer that is used to dilute the samples). We believe the enhancer increases the signal by helping increase the recognition and binding of the target protein by the antibodies in the kit. 

 

Last updated: March 19, 2024