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Which phospho-AMPKα antibody do you suggest for my western blot experiment?

The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:

  • Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 
  • Phospho-AMPKα (Thr172) Antibody #2531
  • Phospho-AMPKα (Thr172) (D4D6D) Rabbit mAb #50081
  • Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb #4188 

The Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 specifically detects endogenous AMPKα1 only when phosphorylated at Thr183, and endogenous AMPKα2 when phosphorylated at Thr172. The other antibodies, #2531, #50081, and #4188, detect both isoforms α1 and α2 when phosphorylated at Thr172. None of these antibodies detect the regulatory β or γ subunits. 

A side-by-side comparison of these antibodies has been performed. It demonstrates that the rabbit monoclonal antibodies, #50081 and #4188, demonstrate the strongest signal strength in western blotting applications. The antibody with the weakest signal strength by western blotting is the rabbit polyclonal antibody #2531.

If you would like more information or to obtain this comparative data, please do not hesitate to reach out to one of our Technical Support scientists

Last updated: July 1, 2024

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