Which species does the Caspase-3 Activity Assay Kit #5723 work in?

We have validated the Caspase-3 Activity Assay Kit #5723 in human and mouse cell lines. However, as long as there is active caspase-3 present, this kit is predicted to work with most cell lines. Depending on the cell type and the incubation time applied in the assay, 0.5 - 2x10^5 cells/well (or 100 ug/well of total lysate protein) is sufficient for most experimental setups. For best results, cell number or lysate concentration titrations are recommended. Because caspase-7 shares the same substrate sequence as caspase-3, this kit also detects caspase-7 activity.

Last updated: February 28, 2024