Why am I not getting a signal for NRF2 in my experiment?

NRF2 can be a tricky protein to work with. Under basal conditions, the protein is marked for ubiquitin-mediated degradation in the cytoplasm and is therefore difficult to capture in the cell lysate. In order to detect this target in cell lysates, we typically treat the cells with a compound or chemical modulator to prevent the degradation of NRF2. For QC purposes, we treat cells with MG-132, #2194, a proteasome inhibitor, which helps prevent degradation and allows for accumulation of NRF2 within the cell. 

Last updated: September 12, 2024