Why am I seeing morphology changes and loss of cells after adding the fixing/denaturing solution from the BrdU Cell Proliferation Assay Kit?

While performing the BrdU cell proliferation assay, we also notice some changes in cell morphology after adding the fixing/denaturing solution.  However, in our experience, it does not adversely affect the signal obtained with the kit. It should be noted that we do not recommend any washes after fixation/denaturation. Instead, we recommend waiting until after incubating the detection solution for one hour at room temperature. No centrifugation step is required unless starting with suspension cell lines.

Last updated: September 12, 2024