Why are two bands observed when using some of your NDRG1 antibodies?

The NDRG1 target has 3 isoforms produced by alternative splicing (see Q92597). Isoform 2 and isoform 3 are missing amino acids 1-66 and amino acids 1-81, respectively. Depending on the species and cell line/tissue, each of the isoforms could be expressed at varying levels. Different treatments can also lead to varying amounts of isoform expression.

The respective antigens for NDRG1 (D8G9) XP® Rabbit mAb #9485, NDRG1 (D10F8) Rabbit mAb #9395, and NDRG1 Antibody #5196 correspond to residues in the carboxy terminus of human NDRG1 protein. Therefore, #9485, #9395, and #5196 can detect all 3 isoforms, leading to the observation of multiple bands. The antigen for NDRG1 (D6C2) Rabbit mAb #9408, on the other hand, corresponds to residues near the amino terminus and this antibody can only detect isoform 1. 
 

Last updated: February 27, 2024