Why do I detect a large smear when using your B7-H4 (D1M8I) XP® Rabbit mAb #14572 for western blot?

The predicted molecular weight of B7-H4 is 28.8 kDa, but by western blot it is usually observed at 40-80 kDa. This is due to glycosylation of the protein. It is common for a target to shift to higher molecular weights with post-translational modification (e.g., glycosylation). Please see Salceda, S. (2005) et al. Exp Cell Res. 306, 128-41 (PMID 15878339; http://www.ncbi.nlm.nih.gov/pubmed/15878339).

Last updated: February 26, 2024