Why do I have high numbers of doublets/multiplets in my single-cell data?

The rate at which the methanol fixation buffer is added can impact cell clumping. In Cell Fixation (Day 1) - step 7 of the InTraSeq protocol, it is very important to dispense the methanol over a period of at least 60 seconds. Failure to do so can result in doublets/multiplets.

If cell clumps are still observed after Washes and Count (Day 3) - step 8 of the InTraSeq protocol, additional pipette mixing is recommended before proceeding to the single-cell experiment.

Last updated: September 18, 2024