Why do I need to use the three buffers in the Cell Fractionation Kit #9038?

Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell permeability agent, which causes the plasma membrane to become permeable enough to release cytosolic proteins but also leave other organelles intact. The Membrane Isolation Buffer (MIB) contains another non-ionic detergent used to lyse and solubilize membrane proteins. Finally, the Cytoskeletal/Nuclear Isolation Buffer (CyNIB) contains a denaturing detergent. The specificity of each buffer allows the user to generate strong, clean fractions.  

Last updated: September 12, 2024