Why do I see two bands at 50 and 65kDa when using the HPSE (E3N3H) Rabbit mAb #99756 for western blot?

HPSE is synthesized as a 65 kDa inactive precursor that undergoes proteolytic processing, yielding 8 kDa and 50 kDa protein subunits that heterodimerize to form an active enzyme. The 65kDa proform and the 50 kDa subunit (amino acids 158-543) are detected by the HPSE (E3N3H) Rabbit mAb #99756, while the 8kDa subunit (amino acids 36-109) is not.

Last updated: April 16, 2026