Why do your Glucocorticoid Receptor (D6H2L) XP® #12041 and Glucocorticoid Receptor (D8H2) XP® #3660 Rabbit mAbs detect two bands at 91 and 94 kDa?

From our testing, we have seen that our Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. It is possible that #12041 may detect other less common isoforms as well, but we have not confirmed this experimentally. Additionally, each of the isoforms that can be detected most likely have post-translational modifications that affect the molecular weight.

Our Glucocorticoid Receptor (D8H2) XP® Rabbit mAb #3660 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. However, based on sequence alignment (and unlike #12041) #3660 is predicted to cross-react with all known alternative translation start site generated isoforms of glucocorticoid receptor-α and glucocorticoid receptor-β (80 kDa).

Please refer to Uniprot ID: P04150 for the reference sequences of both alpha and beta isoforms of the Glucocorticoid Receptor. 

Last updated: April 1, 2024