Why does the observed size versus the theoretical size of TET2 differ by western blot?

TET2 is post-translationally modified (https://www.phosphosite.org/proteinAction.action?id=22594&;showAllSites=true). Although the theoretical molecular weight is 223.8 kDa these modifications increase the molecular weight so that it is usually observed at ~ 280 kDa. Comparison testing has been performed with  our TET2 (D6C7K) Rabbit mAb (Mouse Specific) #36449 and TET2 Antibody #45010 (approved for human, mouse, and monkey) and the bands migrate at the same molecular weight for both antibodies. The specificity of #45010 was tested with HEK parental and TET2 knock-out cell lines (see datasheet) and the observed target band was confirmed to be TET2. This validates the observed band size that we see with our TET2 antibodies, including #36449.

Last updated: February 28, 2024