Why does the signal detected with your Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb #2537 decrease with AMPK activation?

The Ser485 site detected by our Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb #2537 has been identified as an inhibitory phosphosite of APMKα1 and will be phosphorylated to a lesser degree when AMPK is activated in response to nutrient-deprived conditions (e.g., serum or glucose starvation). Therefore, you should see a decrease in signal intensity with #2537 in serum-starved samples, such as the positive extract included in our AMPK Control Cell Extracts #9158.

Last updated: February 23, 2024