Why does your AMPKα Antibody #2532 detect bands at approximately 50 kDa?

While we know from our experiments that our AMPKα Antibody #2532 can detect both the alpha1 and alpha2 isoforms of the catalytic subunit, these should both run above 60kDa. We do occasionally see some non-specific banding around 50 kDa or lower. However, this appears to be very sample dependent and can often be titrated out by loading less lysate. The specific AMPKα signal should be a prominent band around 62kDa.

Last updated: September 12, 2024