Why doesn't PD-L1 migrate at 33 kDa?

The predicted molecular weight of PD-L1 is 33 kDa (based on amino acid composition). However, in most models, PD-L1 migrates at an observed molecular weight of 40-50 kDa on Tris-Glycine gels. This discrepancy between the predicted and the observed molecular weights is a result of glycosylation [see Li, C. et al. (2016) Nat. Commun. 7:12632 (PMID: 27572267; https://www.ncbi.nlm.nih.gov/pubmed/27572267)].

Last updated: October 2, 2024