Why doesn't PD-L2 migrate at 31 kDa?

The predicted molecular weight of PD-L2 is 31 kDa (based on amino acid composition). However, in most models, PD-L2 migrates at an observed molecular weight of 45-60 kDa on Tris-Glycine gels. This discrepancy between the predicted and the observed molecular weights is a result of glycosylation [see Wang, H et al. (2017) Oncoimmunology 6(7), e1327494 (PMID: 28811964; https://www.ncbi.nlm.nih.gov/pubmed/28811964)].

Last updated: February 28, 2024