Why don't your FGF Receptor 2 antibodies detect a band at the predicted molecular weight of 92 kDa?

While FGF Receptor 2 can migrate at the predicted 92 kDa, due to post-translational N-linked glycosylation at 8 predicted sites, it is often observed at greater than 140 kDa (see https://www.uniprot.org/uniprot/P21802). This variability in migration (for FGFR1, 2, and 3) has been experimentally validated in the literature, often by treating samples with glycosylation inhibitors:

Keegan, K. et al. (1991) Oncogene 6, 2229-36 (PMID: 1662791, https://www.ncbi.nlm.nih.gov/pubmed/1662791)

Hatch, N.E. et al. (2006) J Biol Chem. 281, 27292-305 (PMID: 16844695; https://www.ncbi.nlm.nih.gov/pubmed/16844695)
 

Last updated: September 12, 2024