Why is my signaling pathway of interest observed across all my cells in my InTraSeq™ experiment?

Many signaling pathway targets are ubiquitously expressed and can be detected in multiple cell types. When analyzing and interpreting signaling pathway data, make sure to design your experiments with the appropriate controls (knockdowns, knockouts, stimulation, inhibition, etc.).

Using a color gradient in FeaturePlot helps facilitate the analysis and interpretation of single-cell signaling pathway data. See the example in bold below using blue (low expression), grey (mid expression), and red (high expression):
 

FeaturePlot(your_object, features = c("protein_Phospho-CREB(Ser133)-Ab"), max.cutoff = "q95", min.cutoff = "q5") + scale_color_gradientn(colours = c("#003C7B", "#73A9D2", "grey90", "#FDB393", "#b22222"))

Last updated: July 25, 2025