Will your Ubiquitin antibodies detect free-Ubiquitin?

The Ubiquitin (E4I2J) Rabbit mAb #43124 is the preferred antibody for detecting free-ubiquitin. Ubiquitin (P37) Antibody #58395 is also an option for the detection of free-ubiquitin, while Ubiquitin (P4D1) Mouse mAb #3936 and Ubiquitin (E6K4Y) XP® Rabbit mAb #20326 detect free-ubiquitin weakly. To increase the signal for free-ubiquitin (and all ubiquitin levels), the membrane can be boiled for 30 minutes or autoclaved for 30 minutes on a wet cycle prior to the blocking step.

Last updated: February 21, 2024