Context is Key: Leveraging Spatial Biology in Immunology and Immunotherapy

It is known that the immune system plays a role in a variety of disease. This is leading to increased efforts to apply immunotherapy approaches across a growing number of indications. To understand how the immune system contributes to disease progression and to identify potential immunotherapy targets, it is necessary to observe which immune cell types are present and what functions they are executing. Historically, this was most often accomplished using flow cytometry, but increasingly, scientists are turning to spatial biology techniques because they enable observation of immune cells in their environment. Employing spatial biology techniques such as multiplex IHC (mIHC) provides scientists with information, such as where cells are in a tissue, how they are positioned relative to one another, and if they are organized in specific immune structures such as tertiary lymphoid structures (TLS). In this webinar, you will:

• Learn key considerations for designing mIHC assays including antibody quality, antibody format, panel selection.
• Discover methods available for low-, mid-, and high-plex experiments.
• Review data from example panels designed to address relevant questions related to the role of the immune system in disease.

To explore low-, mid-, and high-plex solutions for mIHC available from CST, please visit the Multiplexing and Spatial Biology Resource Center.