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The Utility of Epitope Tags in Western Blot and Immunoprecipitation

 

Epitope tagging is a technique where a known epitope, consisting of a few amino acids, is fused to a recombinant protein using genetic engineering. Epitope tags can be used for labeling and detection of proteins in immunoassays, and are especially useful when studying newly discovered proteins to which antibodies are not yet available, proteins that generate a poor immune response, or low abundance proteins. This Learning Lab LIVE! presentation and Q&A session focuses on how epitope tags can be combined with antibodies in Western Blot (WB) and Immunoprecipitation (IP) experiments.

Watch the previous Learning Lab LIVE! session: Validating Protein Interactions with Co-Immunoprecipitation Using Endogenous and Tagged Protein Models.