How to Sonicate Chromatin: Sonication ChIP Protocol

Chromatin fragmentation is a crucial step in the ChIP Sonication protocol. Optimizing sonication time for ideal chromatin fragment size, and following some general tips such as keeping the sample on ice, can improve your results. This video covers the sonication protocol, in which sonication facilitates both cell and nuclear lysis, and fragmentation of the chromatin.

Using the Enzymatic ChIP Protocol instead? Watch the companion video.