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ApoB (F8V5F) Rabbit Monoclonal Antibody #10067

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 500
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ApoB (F8V5F) Rabbit Monoclonal Antibody recognizes endogenous levels of total ApoB protein. This antibody detects an 85 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr1511 of human ApoB protein.

    Background

    Apolipoprotein B (ApoB) is a large, amphipathic glycoprotein found in lipoprotein vesicles. Two distinct isoforms, ApoB-48 and ApoB-100, are produced from the APOB gene via post-transcriptional editing. ApoB-48 is synthesized in small intestine enterocytes and secreted into circulation; it is essential for producing and transporting chylomicron lipoprotein. ApoB-100 is synthesized in liver hepatocytes and secreted into circulation; it is required for the production of very-low-density lipoproteins (VLDL) (1). In addition to ApoB, cholesteryl esters and triglycerides are the two other essential structural components of VLDL. Once in circulation, VLDL is further delipidated through lipolysis into intermediate-density lipoprotein (IDL) and low-density lipoprotein (LDL) (2). The deposition of these circulating apoB-containing particles to the arterial wall initiates and drives the atherosclerotic process (3,4). Consequently, evaluating circulating ApoB levels is a highly effective method for assessing atherosclerotic cardiovascular disease (ASCVD) risk (5,6).

    Alternate Names

    Apo B-100; Apo B-48; APOB; apoB-100; apoB-48; apolipoprotein B; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B-100; Apolipoprotein B-48; apolipoprotein B48; FCHL2; FLDB; LDLCQ4

    For Research Use Only. Not for Use in Diagnostic Procedures.
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