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Pan-TEAD (D3F7L) Rabbit Monoclonal Antibody (Biotinylated) #10511

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  • WB

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 50, 53, 55, 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is the biotinylated version of the unconjugated Pan-TEAD (D3F7L) Rabbit Monoclonal Antibody #13295 and is expected to exhibit the same species cross-reactivity. The concentration of the biotinylated antibody is 500 ug/ml. Peptide ELISA data were generated using the biotinylated antibody. Western blot data were generated using the unconjugated antibody.
    MW (kDa) 50, 53, 55, 60

    Product Usage Information

    Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.

    Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.
    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Pan-TEAD (D3F7L) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total TEAD proteins. This antibody has been shown to recognize TEAD 1, 2, 3 and 4 in transfected cell extracts.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly54 of human TEAD1 protein.

    Background

    The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of Yes-associated protein (YAP), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). When the Hippo pathway is turned on, YAP is phosphorylated by LATS1/2 kinase and sequestered in the cytoplasm by 14-3-3 protein binding, rendering YAP inactive. When the Hippo pathway is off, non-phosphorylated YAP translocates to the nucleus where it associates with various transcription factors including members of the transcriptional enhancer factor (TEF) family, also known as the TEA domain (TEAD) family (TEAD1-4) (5,6). Although widely expressed in tissues, the TEAD family proteins have specific tissue and developmental distributions. YAP/TEAD complexes regulate the expression of genes involved in cell proliferation and apoptosis (5).

    Alternate Names

    AA; atrophia areata, peripapillary chorioretinal degeneration; DTEF-1; EFTR-2; ETF; ETFR-1; hRTEF-1B; MGC9014; NTEF-1; Protein GT-IIC; REF1; related transcription enhancer factor 1B; RTEF1; SV40 transcriptional enhancer factor; TCF-13; TCF13; TCF13L1; TEA domain family member 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEA domain family member 2; TEA domain family member 3; TEA domain family member 4; TEA domain family member 5; TEA domain transcription factor 1; TEA domain transcription factor 2; TEA domain transcription factor 3; TEA domain transcription factor 4; TEAD-1; TEAD-2; TEAD-3; TEAD-4; TEAD1; TEAD2; TEAD3; TEAD4; TEAD5; TEF-1; TEF-3; TEF-4; TEF-5; TEF1; TEF3; TEF4; TEF5; TEFR-1; Transcription factor 13; Transcription factor 13-like 1; Transcription factor RTEF-1; transcriptional enhancer factor 1; transcriptional enhancer factor 1-related; transcriptional enhancer factor 3; transcriptional enhancer factor 5; Transcriptional enhancer factor TEF-1; Transcriptional enhancer factor TEF-3; Transcriptional enhancer factor TEF-4; Transcriptional enhancer factor TEF-5; Transcriptional enhancer factor TEF-5 (DTEF-1)

    For Research Use Only. Not for Use in Diagnostic Procedures.
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