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ADA2B (F6G5T) Rabbit Monoclonal Antibody #10691

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  • WB

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 55
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ADA2B (F6G5T) Rabbit Monoclonal Antibody recognizes endogenous levels of total ADA2B protein. This antibody does not cross-react with ADA2A protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp172 of human ADA2B protein.

    Background

    ADA2A and ADA2B are human homologs of the yeast SAGA complex member Ada2. While highly related, ADA2A and B appear functionally distinct. ADA2A is incorporated into PCAF-containing complexes, while ADA2B is recruited to SAGA complexes with GCN5 and Brg1 (1-3). ADA2 proteins form a trimer with GCN5L and ADA3, and enhance GCN5L acetyltransferase activity (4,5). The C-terminal domain of ADA2A and ADA2B determines incorporation into their various complexes, resulting in specific targeting toward histone H3 or H4 (6).

    Alternate Names

    ADA2-beta; ADA2-like protein beta; ADA2(beta); ADA2B; MGC21874; TAD2B; TADA2B; Transcriptional adapter 2-beta; transcriptional adaptor 2 (ADA2 homolog, yeast)-beta; transcriptional adaptor 2B

    For Research Use Only. Not for Use in Diagnostic Procedures.
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