Product Pathways - Development
PhosphoPlus® β-Catenin (Ser675) Antibody Duet #11887
|11887S||1 Kit (10 western blots)||---||In Stock||---|
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|Duet Includes||Quantity||Applications||Reactivity||Homology†||Mol. Wt.||Isotype|
|Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176||100 µl||W, IP, IF-F, IF-IC||Human||Mouse, Rat, Chicken, Xenopus, Zebrafish||92 kDa||Rabbit IgG|
|β-Catenin (D10A8) XP® Rabbit mAb #8480||100 µl||W, IP, IHC-P, IHC-F, IF-F, IF-IC, F, ChIP||Human, Mouse, Rat, Monkey||Zebrafish, Bovine, Pig, Guinea Pig, Horse||92 kDa||Rabbit IgG|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), F=Flow Cytometry, ChIP=Chromatin IP
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
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- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
For Research Use Only. Not For Use In Diagnostic Procedures.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.