Product Pathways - Cell Cycle / Checkpoint
Aurora A (1F8) Mouse mAb #12100
|12100S||100 µl (10 western blots)||---||In Stock||---|
|12100||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||48||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Aurora A (1F8) Mouse mAb recognizes endogenous levels of total Aurora A protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human Aurora A protein.
Western blot analysis of extracts from various cell lines using Aurora A (1F8) Mouse mAb.
Flow cytometric analysis of Jurkat cells using Aurora A (1F8) Mouse mAb and propidium iodide (DNA content). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.
Confocal immunofluorescent analysis of HeLa cells using Aurora A (1F8) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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