Product Pathways - Cytoskeletal Signaling
Atlastin-1 (D2E6) Rabbit mAb #12728
|12728S||100 µl (10 western blots)||---||In Stock||---|
|12728||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||55||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Atlastin-1 (D2E6) Rabbit mAb recognizes endogenous levels of total atlastin-1 protein. This antibody does not cross-react with atlastin-2 or atlastin-3. This antibody may cross-react with a protein of unknown origin at 100 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human atlastin-1 protein.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human atlastin-1 (hAlastin-1-Myc/DDK; +), atlastin-2 (hAlastin-2-Myc/DDK; +) or atlastin-3 (hAlastin-3-Myc/DDK; +) protein, using Atlastin-1 (D2E6) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).
Atlastin proteins are highly conserved members of the dynamin superfamily of membrane GTPases that are involved in the formation of vesicles for the endocytotic and secretory processes (1). Atlastins are required to establish and maintain the morphology of the tubular endoplasmic reticulum (ER) and are therefore important in ER function (2). GTP hydrolysis and dimerization are required for atlastin-dependent ER membrane fusion (3).
Atlastin GTPase 1 (ATL1) is primarily expressed in brain, while the related atlastin 2 and atlastin 3 proteins are ubiquitously expressed (4). Mutations in the atlastin 1 gene SPG3A and the ER defects that result are thought to cause one form of hereditary spastic paraplegia (HSP), a group of heterogeneous neurological disorders characterized by severe progressive spasticity of the lower limbs (5,6).
- McNiven, M.A. et al. (2000) Trends Biochem Sci 25, 115-20.
- Park, S.H. and Blackstone, C. (2010) EMBO Rep 11, 515-21.
- Moss, T.J. et al. (2011) Proc Natl Acad Sci U S A 108, 11133-8.
- Rismanchi, N. et al. (2008) Hum Mol Genet 17, 1591-604.
- Salinas, S. et al. (2008) Lancet Neurol 7, 1127-38.
- Hu, J. et al. (2009) Cell 138, 549-61.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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