Product Pathways - Chromatin Regulation / Epigenetics
HMGN1 (D1I5O) Rabbit mAb #12734
|12734S||100 µl (10 western blots)||---||In Stock||---|
|12734||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||18||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
HMGN1 (D1I5O) Rabbit mAb recognizes endogenous levels of total HMGN1 protein. This antibody does not cross-react with other HMGN proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val32 of human HMGN1 protein.
Western blot analysis of extracts from various cell lines using HMGN1 (D1I5O) Rabbit mAb.
Immunoprecipitation of HMGN1 from HeLa cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or HMGN1 (D1I5O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HMGN1 (D1I5O) Rabbit mAb.
Confocal immunofluorescent analysis of NCCIT cells using HMGN1 (D1I5O) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
High mobility group (HMG) proteins are a superfamily of abundant and ubiquitous nuclear proteins that bind DNA without sequence specificity and induce structural changes to the chromatin fiber to regulate access to the underlying DNA. The HMGN family of proteins, which includes five members (HMGN1-5), is characterized by the presence of several conserved protein domains: a positively charged domain, a nucleosome binding domain, and an acidic C-terminal chromatin-unfolding domain (1,2). HMGN proteins function in transcriptional regulation and are recruited to gene promoters by transcription factors, such as estrogen receptor α (ERα), serum responsive factor (SRF), and PITX2, where they can facilitate either gene activation or repression (3-5). HMGN proteins bind specifically to nucleosomal DNA and reduce compaction of the chromatin fiber, in part by competing with linker histone H1 for nucleosome binding (6). In addition, HMGN proteins act to modulate local levels of post-translational histone modifications, decreasing phosphorylation of histone H3 at Ser10 and histone H2A at Ser1 and increasing acetylation of histone H3 at Lys14 (7-9). HMGN proteins can also modulate the activity of several chromatin-remodeling factors and restrict nucleosome mobility (10).
- Hock, R. et al. (2007) Trends Cell Biol 17, 72-9.
- Gerlitz, G. Biochim Biophys Acta 1799, 80-5.
- Zhu, N. and Hansen, U. (2007) Mol Cell Biol 27, 8859-73.
- Amen, M. et al. (2008) Nucleic Acids Res 36, 462-76.
- Belova, G.I. et al. (2008) J Biol Chem 283, 8080-8.
- Catez, F. et al. (2002) EMBO Rep 3, 760-6.
- Lim, J.H. et al. (2005) EMBO J 24, 3038-48.
- Lim, J.H. et al. (2004) Mol Cell 15, 573-84.
- Postnikov, Y.V. et al. (2006) Biochemistry 45, 15092-9.
- Rattner, B.P. et al. (2009) Mol Cell 34, 620-6.
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