Product Pathways - TGF-beta/Smad Signaling
Smad2/3 Antibody Sampler Kit #12747
|12747S||1 Kit (6 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108||40 µl||W||H, M, R, Mi||60||Rabbit IgG|
|Smad2 (D43B4) XP® Rabbit mAb #5339||40 µl||W, IP, IF-IC, F, ChIP||H, M, R, Mk||60||Rabbit IgG|
|Smad2/3 (D7G7) XP® Rabbit mAb #8685||40 µl||W, IP, IF-IC, F, ChIP||H, M, R, Mk||52, 60||Rabbit IgG|
|Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520||40 µl||W, IP, ChIP||H, M, R||Mk, X, Z, B||52||Rabbit IgG|
|Smad3 (C67H9) Rabbit mAb #9523||40 µl||W, IP, IF-IC, F, ChIP||H, M, R, Mk||X, Z, B||52||Rabbit IgG|
|Smad4 Antibody #9515||40 µl||W, ChIP||H, M, R, Mk||70||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP
Reactivity Key: H=Human, M=Mouse, R=Rat, Mi=Mink, Mk=Monkey
Western blot analysis of extracts from untreated (-) or TGF-β treated (+) HeLa and NIH/3T3 cells using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) or Smad2 Antibody #3102 (lower).
Western blot analysis of extracts from various cell lines using Smad2 (D43B4) XP® Rabbit mAb #5339.
Western blot analysis of extracts from HeLa and ACHN cells using Smad2/3 (D7G7) XP® Rabbit mAb #8685.
Western blot analysis of extracts from various cell lines using Smad4 Antibody #9515.
The Smad2/3 Antibody Sampler Kit provides an economical means of detecting target proteins of the TGF-β signaling pathway. The kit contains enough primary and secondary antibodies to perform four western blots with each antibody.
Specificity / Sensitivity
Each antibody in the Smad2/3 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site. The total Smad2, Smad3, and Smad4 antibodies detect their respective targets at endogenous levels.
Source / Purification
Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser465/467 of human Smad2 and Ser423/425 of human Smad3. Total Smad2 and Smad3 monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino termini of mouse Smad2 and Smad3 or His198 of human Smad2/3 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Pro278 of human Smad4. Antibodies are purified by protein A and peptide affinity chromatography.
Transforming growth factor-β (TGF-β) superfamily signaling plays a critical role in the regulation of cell growth, differentiation, and development in a wide range of biological systems. In general, signaling is initiated with ligand-induced oligomerization of serine/ threonine receptor kinases and phosphorylation of the cytoplasmic signaling molecules Smad2 and Smad3 for the TGF-β/activin pathway, or Smad1/5/8 for the bone morphogenetic protein (BMP) pathway. Carboxy-terminal phosphorylation of Smad proteins by activated receptors results in their partnering with the common signaling transducer Smad4, and translocation to the nucleus. Activated Smad proteins regulate diverse biological effects by partnering with transcription factors resulting in cell-state specific modulation of transcription (1-4).
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- 12052 Smad2/3 Control Cell Extracts
- 8915 Human Transforming Growth Factor β1 (hTGF-β1)
- 5231 Mouse Transforming Growth Factor β1 (mTGF-β1)
- 11979 Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (PE Conjugate)
- 3103 Smad2 (L16D3) Mouse mAb
- 12470 Smad2/3 (D7G7) XP® Rabbit mAb (Biotinylated)
- 12001 PathScan® Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) Sandwich ELISA Kit
- 12791 SignalSilence® Smad4 siRNA I (Mouse Specific)
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.