Product Pathways - Cell Cycle / Checkpoint
Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb #12958
|12958S||100 µl (10 western blots)||---||In Stock||---|
|12958||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Hamster, Monkey, Dog||Endogenous||125||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Xenopus.
Specificity / Sensitivity
Phospho-MCM2 (Ser139) (D1Z8X) XP® recognizes endogenous levels of MCM2 protein only when phosphorylated at Ser139.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser139 of human MCM2 protein.
Western blot analysis of HeLa cell extracts, untreated (-) or calf intestinal phosphatase (CIP) and λ phosphatase-treated (+), and extracts of T98G cells serum-starved for 48 hours (-) or serum starved for 48 hours followed by serum-stimulation for 24 hours (+), using Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb (upper), MCM2 (D7G11) XP® Rabbit mAb #3619 or α-Actinin (D6F6) XP® Rabbit mAb #6487.
Immunoprecipitation of phospho-MCM2 (Ser139) from T98G cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb.
Confocal immunofluorescent analysis of WI-38 cells, serum-deprived (48 hr; upper left), or serum-treated (10%, 24 hr; upper right) and HeLa cells, untreated (lower left), or λ phosphatase-treated (2 hr; lower right), using Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). CDK1 phosphorylation of MCM3 at Ser112 during late mitosis/early G1 phase has been shown to initiate complex formation and chromatin loading in vitro (8). Phosphorylation of MCM2 at serine 139 by cdc7/dbf4 coincides with the initiation of DNA replication (9). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,10).
- Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.
- Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.
- Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.
- Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.
- Labib, K. et al. (2000) Science 288, 1643-7.
- Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.
- Masai, H. et al. (2006) J Biol Chem 281, 39249-61.
- Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.
- Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
- Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3619 MCM2 (D7G11) XP® Rabbit mAb
- 12686 Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb
- 4003 MCM3 (D47B6) Rabbit mAb
- 3735 MCM7 (D10A11) XP® Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
Tween® is a registered trademark of ICI Americas, Inc.