Product Pathways - Cytoskeletal Signaling
Kinectin 1 (D5F7J) Rabbit mAb #13243
|13243S||100 µl (10 western blots)||---||In Stock||---|
|13243||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey, Dog||Endogenous||160||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Kinectin 1 (D5F7J) Rabbit mAb recognizes endogenous levels of total kinectin 1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val186 of human kinectin 1 protein.
Western blot analysis of extracts from T24, HeLa, and COS-7 cells using Kinectin 1 (D5F7J) Rabbit mAb.
Immunoprecipitation of kinectin 1 from T24 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Kinectin 1 (D5F7J) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Kinectin 1 (D5F7J) Rabbit mAb.
Confocal immunofluorescent analysis of SK-OV-3 cells using Kinectin 1 (D5F7J) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Kinectin 1 (KTN1) is an endoplasmic reticulum (ER)-enriched integral membrane protein that may be involved in the formation of ER sheets (reviewed in 1). Kinectin 1 binds the microtubule motor protein kinesin and acts as a membrane anchor for kinesin-based organelle trafficking (2). The interaction of kinesin with kinectin 1 has been shown to affect ER-supported focal adhesion assembly (3). Kinectin 1 has also been implicated in translation elongation, as an anchor for the elongation factor complex to the ER (4,5). Research investigators have shown that kinectin 1 expression is altered in multiple human pathologies, including breast cancer (6), hepatocellular carcinoma (HCC) (7), Parkinson's disease (8), and the autoimmune syndrome Behçet's disease (BD) (9,10).
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- Kumar, J. et al. (1995) Science 267, 1834-7.
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- Ong, L.L. et al. (2003) J Biol Chem 278, 32115-23.
- Ong, L.L. et al. (2006) J Biol Chem 281, 33621-34.
- Sutton, C.W. et al. (2010) J Proteome Res 9, 3891-902.
- Wang, H.C. et al. (2004) Biochem Cell Biol 82, 321-7.
- van Dijk, K.D. et al. (2012) Brain Pathol 22, 485-98.
- Lu, Y. et al. (2005) Arthritis Res Ther 7, R1133-9.
- Feng, X.G. et al. (2007) Clin Exp Rheumatol 25, S80-5.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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Tween® is a registered trademark of ICI Americas, Inc.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.