CNOT3 (E1L9S) Rabbit Monoclonal Antibody #13300
- WB
- IP
- eCLIP
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 105 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| eCLIP | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Research studies indicate that CNOT3 (along with CNOT1 and CNOT2) represses early developmental transcription factor expression, helping to maintain embryonic stem (ES) cell identity in mice and humans (4). Additional studies suggest that CNOT3 plays a role in mitotic progression as it destabilizes mitotic spindle assembly protein MAD1 mRNA (5). Finally, CNOT3 appears to act as a modifier gene affecting the penetrance of mutations causing autosomal dominant retinitis pigmentosa (6) and as a tumor suppressor associated with cases of adult T-cell acute lymphoblastic leukemia (7).
- Denis, C.L. and Chen, J. (2003) Prog Nucleic Acid Res Mol Biol 73, 221-50.
- Lau, N.C. et al. (2009) Biochem J 422, 443-53.
- Chen, C. et al. (2011) Biochem Biophys Res Commun 411, 360-4.
- Zheng, X. et al. (2012) Stem Cells 30, 910-22.
- Takahashi, A. et al. (2012) Biochem Biophys Res Commun 419, 268-73.
- Venturini, G. et al. (2012) PLoS Genet 8, e1003040.
- De Keersmaecker, K. et al. (2013) Nat Genet 45, 186-90.
Alternate Names
CCR4-associated factor 3; CCR4-NOT transcription complex subunit 3; CCR4-NOT transcription complex, subunit 3; CNOT3; IDDSADF; KIAA0691; LENG2; Leukocyte receptor cluster member 2; NOT3; NOT3 (negative regulator of transcription 3, yeast) homolog; NOT3H
Limited Uses
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