Product Pathways - PI3K / Akt Signaling
Mios (D12C6) Rabbit mAb #13557
|13557S||100 µl (10 western blots)||---||In Stock||---|
|13557||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||98||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Mios (D12C6) Rabbit mAb recognizes endogenous levels of total Mios protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu730 of human Mios protein.
Western blot analysis of extracts from various cell lines using Mios (D12C6) Rabbit mAb.
The mTORC1 kinase complex plays a critical role in cell growth regulation (1,2). mTORC1 activity is modulated by energy levels, growth factors, and amino acids (3,4). Four related GTPases (RagA, RagB, RagC, and RagD) interact with raptor in mTORC1, which is necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). The GAP activity towards Rags (GATOR) complex interacts with Rag GTPases and is made up of a pair of protein subcomplexes (5). The GATOR1 subcomplex includes the proteins DEPDC5, Nprl2 and Nprl3, and is a RagA and RagB GTPase-activating protein (GAP) that negatively regulates mTORC1 signaling. Conversely, the GATOR2 subcomplex (including Mios, WDR24, WDR59, Seh1L and Sec13 proteins) is a positive regulator of mTORC1 signaling (5).
The mammalian Mios protein contains six amino-terminal WD repeats and a carboxy terminal zinc finger-like domain. The homologous Drosophila missing oocyte (Mio) protein is required for meiotic progression and oocyte cell fate maintenance during development (6).
- Sancak, Y. et al. (2008) Science 320, 1496-501.
- Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
- Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
- Wullschleger, S. et al. (2006) Cell 124, 471-84.
- Bar-Peled, L. et al. (2013) Science 340, 1100-6.
- Iida, T. and Lilly, M.A. (2004) Development 131, 1029-39.
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