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Langerin (D9H7R) Rabbit Monoclonal Antibody #13650

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  • WB
  • IP
  • IF
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 40-50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:800
    Flow Cytometry (Fixed/Permeabilized) 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #49830.

    Protocol

    Specificity / Sensitivity

    Langerin (D9H7R) Rabbit Monoclonal Antibody recognizes endogenous levels of total Langerin protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val14 of human Langerin protein.

    Background

    Langerin (CD207) is a C-type lectin receptor whose expression is restricted mainly to dendritic cells in the skin, including Langerhans cells in the epidermis and langerin+/CD103+ dermal dendritic cells (1-4). Langerin is found on the cell surface and within rod-shaped organelles called Birbeck granules, and its expression is required for the formation of Birbeck granules (1,5). Langerin recognizes carbohydrate motifs on the surface of pathogens, resulting in endocytosis of the pathogen into Birbeck granules, degradation of the pathogen, and antigen presentation to T cells (6-8).

    Alternate Names

    C-type lectin domain family 4 member K; C-type lectin domain family 4, member K; CD207; CD207 antigen, langerin; CD207 molecule; CD207 molecule, langerin; CLC4K; CLEC4K; Langerhans cell specific c-type lectin; Langerin

    For Research Use Only. Not for Use in Diagnostic Procedures.
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