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DOG1/Anoctamin 1 (D1M9Q) Rabbit Monoclonal Antibody #14476

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  • IP
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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 150
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DOG1/Anoctamin 1 (D1M9Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total Anoctamin 1 protein. An unidentified band is detected around 30 kDa in some tissues.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala976 of human anoctamin 1 protein.

    Background

    Anoctamin 1 is a plasma membrane calcium-activated chloride channel, also known as discovered on gastrointestinal stromal tumors protein 1 (DOG1) or transmembrane protein 16A (TMEM16A) (1). It is essential for chloride secretion from epithelial tissues (2) and has been shown to be a specific marker for cells of Cajal (3), the pacemaker cells that control smooth muscle contraction. Anoctamin 1 knockout mice exhibit an altered gastric smooth muscle rhythmic contraction (4). More recently, research studies have identified anoctamin 1 as a heat sensor in nociceptive neurons (5). Heat above 44ºC triggers anoctamin 1-dependent depolarization, contributing to the mediation of thermal nociception (5).

    Alternate Names

    ANO1; anoctamin 1; anoctamin 1, calcium activated chloride channel; Anoctamin-1; Ca2+-activated Cl- channel; calcium activated chloride channel; Discovered on gastrointestinal stromal tumors protein 1; DOG1; FLJ10261; oral cancer overexpressed 2; Oral cancer overexpressed protein 2; ORAOV2; TAOS2; TMEM16A; Transmembrane protein 16A; transmembrane protein 16A (eight membrane-spanning domains); tumor amplified and overexpressed sequence 2; Tumor-amplified and overexpressed sequence 2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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