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MATE1/SLC47A1 (D4C6Z) Rabbit Monoclonal Antibody #14550

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 48-52
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MATE1/SLC47A1 (D4C6Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total MATE1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu505 of human MATE1 protein.

    Background

    The multidrug and toxin extrusion protein 1 (MATE1, SLC47A1) is a proton-coupled, organic cation antiporter located at the apical membrane of proximal kidney epithelial cells and the canalicular membrane of hepatocytes (1). MATE1 mediates the secretion of organic cations including drugs, toxins, and endogenous metabolites, into bile and urine (2,3). Substrates of MATE1 include multiple therapeutic agents, including metformin, cisplatin, acyclovir, and cephalexin (4,5). Polymorphisms in the corresponding SLC47A1 gene may affect the rate of renal clearance of certain cationic drugs, limiting the therapeutic benefits of these agents (6). Specifically, research studies demonstrate that SLC47A1 allelic variation correlates with differences in renal clearance rates of metformin (7), which may have an effect on the therapeutic impact of this drug in individuals diagnosed with type 2 diabetes (8).

    Alternate Names

    FLJ10847; hMATE-1; MATE-1; MATE1; MGC64822; multidrug and toxin extrusion 1; Multidrug and toxin extrusion protein 1; S47A1; SLC47A1; solute carrier family 47 (multidrug and toxin extrusion), member 1; Solute carrier family 47 member 1; solute carrier family 47, member 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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