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VCAM-1 Antibody

VCAM-1 Antibody #14694

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Product Specifications

REACTIVITY	M R
SENSITIVITY	Endogenous
MW (kDa)	35-45, 85-100
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

VCAM-1 Antibody recognizes endogenous levels of total VCAM-1 protein. This antibody recognizes both long and short isoforms of VCAM-1 protein.

Species Reactivity:

Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln203 of mouse VCAM-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3).

Alternate Names

CD106; V-CAM 1; vascular cell adhesion molecule 1; Vascular cell adhesion protein 1; Vcam; VCAM-1; Vcam1

Database Links

UniProt ID: P29533

Entrez-Gene Id: 22329

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For Research Use Only. Not for Use in Diagnostic Procedures.

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