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FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody #16563

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  • WB
  • IP
  • F

    Product Specifications

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600
    Flow Cytometry (Live) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody recognizes exogenously expressed levels of CARs containing an FMC63-derived scFv.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with an scFv derived from a murine anti-CD19 monoclonal antibody, FMC63.

    Background

    Chimeric antigen receptor (CAR) T cell therapy utilizes synthetic receptors to redirect T cells to bind and eliminate tumor cells, bypassing traditional T cell receptor (TCR) signaling (1). The extracellular domain of conventional CARs consists of single-chain variable fragments (scFvs), which recognize and bind target antigens (1). CARs engineered to express the scFv of a murine anti-CD19 antibody, FMC63, have shown clinical success in targeting CD19-expressing malignant B cells for degradation. This clinical success has led to several FMC63-based CARs being approved by the U.S. Food and Drug Administration (FDA) for the treatment of B-cell leukemia and lymphoma (2,3).

    Alternate Names

    B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD19; CD19 antigen; CD19 molecule; CVID3; Differentiation antigen CD19; MGC12802; T-cell surface antigen Leu-12

    For Research Use Only. Not for Use in Diagnostic Procedures.
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