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VAMP5 (F9Y8C) Rabbit Monoclonal Antibody #17055

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 13
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VAMP5 (F9Y8C) Rabbit Monoclonal Antibody recognizes endogenous levels of total VAMP5 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VAMP5 protein.

    Background

    Vesicle-associated membrane protein 5 (VAMP5), also known as myobrevin, is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein family, preferentially expressed in skeletal muscle and heart, with increased expression during myogenesis (1). While full functionality of VAMP5 is unknown, it has been shown to mediate exosome release through its interaction with SNAP47, syntaxin1, or syntaxin4 (2). VAMP5 has also been shown to promote Fcγ receptor-mediated phagocytosis and may play a role in regulating macrophage phagosome maturation (3). Plasma levels of VAMP5 are downregulated in Alzheimer’s disease (AD), identifying the protein as a potential blood biomarker for AD when paired with other key differentially expressed proteins (4).

    Alternate Names

    Myobrevin; VAMP-5; VAMP5; vesicle associated membrane protein 5; Vesicle-associated membrane protein 5; vesicle-associated membrane protein 5 (myobrevin)

    For Research Use Only. Not for Use in Diagnostic Procedures.
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