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  3. Phospho-LYN (Tyr397)/LCK (Tyr394)/HCK (Tyr411)/BLK (Tyr389) (E5L3D) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)
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Phospho-LYN (Tyr397)/LCK (Tyr394)/HCK (Tyr411)/BLK (Tyr389) (E5L3D) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) #17320

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    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-LYN (Tyr397)/LCK (Tyr394)/HCK (Tyr411)/BLK (Tyr389) (E5L3D) Rabbit mAb #70926.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    Phospho-LYN (Tyr397)/LCK (Tyr394)/HCK (Tyr411)/BLK (Tyr389) (E5L3D) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) detects endogenous levels of LYN, LCK, HCK, and BLK only when phosphorylated at Tyr397, Tyr394, Tyr411, and Tyr389, respectively. This antibody conjugate may cross-react with overexpressed phosphorylated Src, YES, FYN, and FGR.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human LYN protein.

    Background

    The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

    Alternate Names

    ASV; c-SRC; c-syn protooncogene; c-yes; cellular yes-1 protein; FLJ26625; FYN; FYN oncogene related to SRC, FGR, YES; FYN proto-oncogene, Src family tyrosine kinase; HCK; HCK proto-oncogene, Src family tyrosine kinase; Hematopoietic cell kinase; Hemopoietic cell kinase; HsT441; IMD22; JTK8; JTK9; LCK; LCK proto-oncogene, Src family tyrosine kinase; Lck/Yes-related novel protein tyrosine kinase; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; LYN; LYN proto-oncogene, Src family tyrosine kinase; MGC45350; OKT3-induced calcium influx regulator; p53Lyn; p56-LCK; p56(LSTRA) protein-tyrosine kinase; p56lck; p56Lyn; p59-Fyn; p59-HCK/p60-HCK; p59Hck; p60-Src; p61-Yes; p61Hck; pp58lck; pp60c-src; Protein YT16; protein-tyrosine kinase fyn; Proto-oncogene c-Fyn; Proto-oncogene c-Src; Proto-oncogene c-Yes; Proto-oncogene Lck; Proto-oncogene Syn; proto-oncogene tyrosine-protein kinase fyn; proto-oncogene tyrosine-protein kinase LCK; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene tyrosine-protein kinase Yes; protooncogene SRC, Rous sarcoma; SLK; SRC; SRC proto-oncogene, non-receptor tyrosine kinase; src-like kinase; src/yes-related novel; SRC1; SYN; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; THC6; tyrosine kinase p59fyn(T); tyrosine kinase pp60c-src; Tyrosine-protein kinase Fyn; Tyrosine-protein kinase HCK; Tyrosine-protein kinase Lck; Tyrosine-protein kinase Lyn; tyrosine-protein kinase SRC-1; Tyrosine-protein kinase Yes; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian); v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; viral oncogene yes-1 homolog 1; Yamaguchi sarcoma oncogene; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog; YES; YES proto-oncogene 1, Src family tyrosine kinase; YES1; YES1 proto-oncogene, Src family tyrosine kinase; YT16

    Database Links

    UniProt ID: P07947 , P07948 , P06239 , P08631 , P12931 , P06241


    Entrez-Gene Id: 7525 , 4067 , 3932 , 3055 , 6714 , 2534


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