Phospho-NCK1 (Ser85) (F8P1Y) Rabbit Monoclonal Antibody #18751
- WB
- IP
- F
Product Specifications
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 47 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:50 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
NCK1 is phosphorylated at Ser85 in response to cell stimulation, alleviating an inhibitory loop between the protein's SH3.1 and SH3.2 domains (8,9).
- Lehmann, J.M. et al. (1990) Nucleic Acids Res. 18, 1048.
- Chou, M.M. et al. (1992) Mol. Cell Biol. 12, 5834-5842.
- Li, W. et al. (1992) Mol. Cell Biol. 12, 5824-5833.
- Verma, R. et al. (2006) J. Clin. Invest. 116, 1346-1359.
- Rivera, G.M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9536-9541.
- Li, H. et al. (2006) Biochem. Biophys. Res. Commun. 349, 310-316.
- Bladt, F. et al. (2003) Mol. Cell Biol. 23, 4586-4597.
- Mayya, V. et al. (2009) Sci Signal 2, ra46.
- Konakahara, S. et al. (2012) FEBS Lett 586, 2251-9.
Alternate Names
Cytoplasmic protein NCK1; melanoma NCK protein; MGC12668; NCK; NCK adaptor protein 1; NCK tyrosine kinase; Nck-1; NCK1; NCKalpha; non-catalytic region of tyrosine kinase; SH2/SH3 adaptor protein NCK-alpha
Limited Uses
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