CALCOCO1 (F4P2D) Rabbit Monoclonal Antibody #19004
- WB
- IP
Product Specifications
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 95 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
More recently, mass spectrometry proteomic profiling aimed at identifying proteins in the mTOR-autophagy axis revealed a role for CALCOCO1 in selective autophagy (4). Notably, CALCOCO1 acts as a soluble reticulophagy receptor for the selective degradation of tubular endoplasmic reticulum (ER) in response to proteotoxic and nutrient stress. CALCOCO1-mediated ER-phagy requires interaction with VAMP-associated proteins on the ER membrane via its C-terminal FFAT-like motif, followed by binding to ATG8/LC3 proteins on the autophagosome via its LC3-interacting region (LIR) motif (5). Additionally, CALCOCO1 binds the Golgi-resident palmitoyltransferase ZDHHC17 and ATG8/LC3 proteins to facilitate golgiphagy (6).
- Kim, J.H. et al. (2003) Mol Cell 12, 1537-49.
- Yang, C.K. et al. (2006) J Biol Chem 281, 3389-97.
- Yang, C.K. et al. (2006) Mol Endocrinol 20, 3251-62.
- Stefely, J.A. et al. (2020) Autophagy 16, 2219-2237.
- Nthiga, T.M. et al. (2020) EMBO J 39, e103649.
- Nthiga, T.M. et al. (2021) J Cell Biol 220, e202006128. doi: 10.1083/jcb.202006128.
Alternate Names
CACO1; calcium binding and coiled-coil domain 1; Calcium-binding and coiled-coil domain-containing protein 1; CALCOCO1; Calphoglin; Cocoa; Coiled-coil coactivator protein; coiled-coil leucine zipper coactivator 1; inorganic pyrophosphatase activator; KIAA1536; PP13275; Sarcoma antigen NY-SAR-3
Limited Uses
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